Validation of candidate genes and regulators
Gene candidates for Fluidigm validation
| Symbol | Module | Type | Correlation | Z.score | Min | Max | Mean | Median | Variance | TaqMan.ID | p-value | MA/Flu Correlation | Efficiency |
| ifi204 | 02 | regulator | 0.91 | 6.33 | 2.21 | 9.58 | 3.80 | 2.66 | 5.35 | Mm00492602_m1* | 1.590e-3 | -9.120e-1 | 9.760e-1 |
| maf | 02 | regulator | 0.92 | 6.18 | 3.18 | 9.00 | 5.00 | 4.31 | 2.97 | Mm02581355_s1* | 3.320e-2 | -7.470e-1 | 5.950e-1 |
| irf8 | 02 | regulator | 0.86 | 6.15 | 3.42 | 9.78 | 6.52 | 6.30 | 3.03 | Mm01250091_m1 | 1.220e-2 | -8.230e-1 | 9.660e-1 |
| tifab | 02 | exemplar | 0.97 | 0.00 | 3.17 | 8.32 | 4.48 | 3.89 | 2.49 | Mm04210261_m1* | 1.250e-1 | -5.880e-1 | 1.012e+0 |
| ifnar2 | 03 | regulator | 0.95 | 4.23 | 4.87 | 9.90 | 8.12 | 8.35 | 1.67 | Mm00494916_m1* | 1.890e-1 | -5.170e-1 | 9.170e-1 |
| hcls1 | 03 | regulator | 0.97 | 4.39 | 2.46 | 9.11 | 7.21 | 7.83 | 3.27 | Mm00468528_m1* | 7.450e-1 | 1.380e-1 | 1.960e-1 |
| evi2a | 03 | exemplar | 0.98 | 0.00 | 2.61 | 8.07 | 6.54 | 6.84 | 2.02 | Mm03030107_s1* | 5.800e-2 | -6.900e-1 | 1.124e+0 |
| evi1 | 07 | regulator | 0.87 | 7.05 | 2.92 | 7.89 | 5.56 | 5.33 | 2.49 | Mm00514814_m1* | 4.580e-2 | -7.160e-1 | 8.270e-1 |
| hoxa9 | 07 | regulator | 0.89 | 7.23 | 3.53 | 10.22 | 6.94 | 7.06 | 5.61 | Mm00439364_m1* | 2.460e-5 | -9.780e-1 | 7.530e-1 |
| prdm16 | 07 | regulator | 0.91 | 7.15 | 3.86 | 7.71 | 5.75 | 5.41 | 1.30 | Mm00712556_m1* | 3.290e-3 | -8.870e-1 | 7.750e-1 |
| hoxa10 | 07 | regulator | 0.94 | 7.97 | 2.41 | 5.58 | 3.95 | 3.49 | 1.26 | Mm00433966_m1* | 2.790e-5 | -9.780e-1 | 9.030e-1 |
| eya1 | 07 | exemplar | 0.97 | 0.00 | 3.42 | 7.63 | 4.97 | 4.44 | 1.52 | Mm00438796_m1* | 6.380e-2 | -6.790e-1 | 1.212e+0 |
| myb | 10 | regulator | 0.92 | 5.57 | 4.74 | 11.68 | 9.89 | 10.77 | 3.54 | Mm00501741_m1* | 5.100e-3 | -8.690e-1 | 1.223e+0 |
| mpl | 10 | regulator | 0.91 | 5.64 | 4.68 | 10.34 | 8.24 | 8.68 | 2.74 | Mm00440310_m1* | 3.690e-4 | -9.460e-1 | 1.011e+0 |
| erg | 10 | regulator | 0.95 | 6.62 | 4.59 | 10.10 | 8.12 | 8.56 | 2.63 | Mm00504897_m1 | 5.320e-1 | 2.610e-1 | -1.000e+0 |
| angpt1 | 10 | exemplar | 0.97 | 0.00 | 3.43 | 10.64 | 7.64 | 8.49 | 4.31 | Mm00456503_m1* | 1.070e-3 | -9.230e-1 | 1.128e+0 |
| gimap6 | 11 | exemplar | 0.93 | 0.00 | 3.50 | 11.00 | 7.90 | 8.43 | 4.82 | Mm00462641_m1* | 1.070e-4 | -9.650e-1 | 8.150e-1 |
| nmi | 12 | regulator | 0.89 | 5.72 | 5.15 | 10.40 | 8.47 | 9.08 | 2.14 | Mm00803857_m1* | 6.910e-3 | -8.540e-1 | 1.092e+0 |
| irf9 | 12 | regulator | 0.93 | 7.42 | 4.71 | 9.10 | 7.40 | 7.75 | 1.25 | Mm00492673_m1 | 4.440e-3 | -8.750e-1 | 9.800e-1 |
| ai451617 | 12 | exemplar | 0.97 | 0.00 | 3.43 | 8.44 | 6.00 | 6.42 | 2.15 | Mm01162558_m1* | 1.850e-3 | -9.070e-1 | 1.149e+0 |
| osgin1 | 12 | regulator | 0.89 | 6.13 | 4.27 | 8.09 | 6.71 | 7.17 | 1.11 | Mm00660947_m1* | 7.670e-3 | -8.490e-1 | 8.050e-1 |
| cxcl2 | 17 | exemplar | 0.95 | 0.00 | 2.69 | 13.50 | 5.52 | 3.48 | 12.44 | Mm00436450_m1* | 3.330e-4 | -9.480e-1 | 9.650e-1 |
| fosl2 | 17 | regulator | 0.84 | 7.21 | 5.29 | 9.20 | 6.64 | 6.41 | 0.84 | Mm00484442_m1* | 2.050e-2 | -7.870e-1 | 1.029e+0 |
| rel | 17 | regulator | 0.89 | 7.71 | 3.68 | 9.06 | 5.98 | 5.73 | 1.73 | Mm01239661_m1* | 3.370e-2 | -7.460e-1 | 9.120e-1 |
| nr4a3 | 17 | regulator | 0.91 | 7.52 | 3.83 | 6.91 | 4.54 | 4.28 | 0.44 | Mm00450074_m1* | 4.250e-3 | -8.770e-1 | 1.157e+0 |
| rps6ka4 | 19 | regulator | 0.78 | 6.05 | 3.83 | 7.17 | 5.25 | 5.17 | 0.67 | Mm00451280_m1* | 9.270e-3 | -8.390e-1 | 1.324e+0 |
| tcfe3 | 19 | regulator | 0.87 | 5.56 | 6.40 | 10.55 | 8.32 | 7.94 | 1.16 | Mm01341186_m1* | 1.510e-2 | -8.080e-1 | 1.240e+0 |
| wwp1 | 19 | regulator | 0.86 | 5.40 | 5.98 | 8.51 | 7.07 | 6.98 | 0.34 | Mm01210682_m1* | 2.230e-2 | -7.800e-1 | 1.073e+0 |
| cttnbp2nl | 19 | exemplar | 0.96 | 0.00 | 3.25 | 8.69 | 5.14 | 4.40 | 2.30 | Mm00518765_m1* | 1.460e-2 | -8.110e-1 | 7.770e-1 |
| mc2r | 27 | exemplar | 0.97 | 0.00 | 2.22 | 10.16 | 3.32 | 2.87 | 2.90 | Mm01262510_m1* | 1.610e-6 | -9.910e-1 | 1.021e+0 |
| dpf3 | 27 | regulator | 0.67 | 4.96 | 4.34 | 6.03 | 4.91 | 4.82 | 0.13 | Mm00475440_m1* | 2.750e-3 | -8.940e-1 | 8.710e-1 |
| celsr1 | 29 | exemplar | 0.93 | 0.00 | 2.85 | 7.16 | 4.74 | 4.42 | 1.33 | Mm00464808_m1* | 3.550e-2 | -7.410e-1 | 9.770e-1 |
| b930041f14rik | 29 | regulator | 0.84 | 6.83 | 4.21 | 7.50 | 5.88 | 5.80 | 0.74 | Mm00844649_s1* | 5.230e-2 | -7.020e-1 | 7.510e-1 |
| pml | 29 | regulator | 0.87 | 5.43 | 5.32 | 8.76 | 6.57 | 6.35 | 0.77 | Mm00476969_m1* | 6.830e-1 | -1.730e-1 | 1.146e+0 |
| klf12 | 29 | regulator | 0.84 | 6.12 | 3.17 | 5.47 | 4.20 | 4.12 | 0.32 | Mm00516098_m1* | 1.790e-2 | -7.970e-1 | 1.033e+0 |
| zdhhc16 | 38 | regulator | 0.75 | 4.26 | 7.62 | 9.12 | 8.52 | 8.57 | 0.13 | Mm00470108_m1* | 7.980e-2 | -6.520e-1 | 1.085e+0 |
| zdhhc21 | 38 | regulator | 0.78 | 4.39 | 6.30 | 8.80 | 7.54 | 7.64 | 0.35 | Mm00509795_m1* | 4.400e-2 | -7.200e-1 | 7.510e-1 |
| pip4k2a | 38 | exemplar | 0.94 | 0.00 | 5.71 | 9.51 | 7.78 | 8.11 | 0.77 | Mm00435721_m1* | 6.460e-1 | -1.940e-1 | 5.600e-1 |
| foxn2 | 38 | regulator | 0.78 | 4.74 | 5.48 | 8.32 | 6.91 | 6.83 | 0.49 | Mm00839106_g1 | 8.000e-1 | -1.080e-1 | 8.820e-1 |
| esr1 | 39 | regulator | 0.95 | 7.94 | 2.71 | 7.13 | 3.40 | 3.10 | 0.99 | Mm00433149_m1* | 6.720e-3 | -8.560e-1 | 9.760e-1 |
| zbtb20 | 39 | regulator | 0.88 | 6.51 | 3.72 | 7.13 | 4.75 | 4.57 | 0.57 | Mm00457765_m1* | 1.060e-2 | -8.310e-1 | 1.186e+0 |
| aldh1a1 | 39 | exemplar | 0.96 | 0.00 | 2.50 | 10.55 | 3.51 | 2.91 | 3.71 | Mm00657317_m1* | 2.980e-6 | -9.890e-1 | 8.940e-1 |
| foxa3 | 39 | regulator | 0.88 | 6.50 | 3.25 | 7.85 | 4.55 | 4.31 | 1.26 | Mm00484714_m1* | 4.910e-3 | -8.710e-1 | 1.049e+0 |
| cebpa | 40 | regulator | 0.87 | 6.76 | 3.36 | 9.56 | 6.35 | 6.17 | 2.95 | Mm00514283_s1* | 1.750e-1 | -5.320e-1 | 1.218e+0 |
| slc16a10 | 40 | exemplar | 0.91 | 0.00 | 4.24 | 7.37 | 5.88 | 5.95 | 0.85 | Mm00661045_m1* | 5.430e-3 | -8.660e-1 | 9.020e-1 |
| ddx58 | 42 | regulator | 0.81 | 6.05 | 3.95 | 7.29 | 5.75 | 5.86 | 0.60 | Mm00554529_m1* | 1.050e-1 | -6.150e-1 | 1.197e+0 |
| ldhb | 42 | exemplar | 0.93 | 0.00 | 6.53 | 12.67 | 9.52 | 9.82 | 2.36 | Mm00493146_m1* | 8.190e-2 | -6.490e-1 | 1.400e+0 |
| dedd2 | 42 | regulator | 0.70 | 5.76 | 5.14 | 7.47 | 6.57 | 6.60 | 0.38 | Mm01149726_m1* | 2.690e-1 | -4.450e-1 | 1.094e+0 |
| hoxd8 | 43 | regulator | 0.90 | 7.49 | 3.26 | 7.85 | 4.30 | 3.87 | 1.29 | Mm03016337_m1* | 3.330e-3 | -8.870e-1 | 8.030e-1 |
| reln | 43 | exemplar | 0.96 | 0.00 | 2.85 | 7.36 | 3.69 | 3.28 | 1.12 | Mm00465200_m1* | 2.270e-6 | -9.900e-1 | 8.120e-1 |
| hoxd1 | 43 | regulator | 0.95 | 8.64 | 2.06 | 7.19 | 3.03 | 2.56 | 1.87 | Mm00439370_g1* | 1.440e-4 | -9.610e-1 | 9.450e-1 |
| nubp1 | 44 | exemplar | 0.92 | 0.00 | 6.35 | 8.66 | 7.51 | 7.49 | 0.33 | Mm00478752_m1* | 4.220e-1 | -3.320e-1 | 1.134e+0 |
| gtf2f2 | 44 | regulator | 0.83 | 6.08 | 7.83 | 10.64 | 9.17 | 9.10 | 0.49 | Mm01310683_m1* | 2.190e-1 | -4.890e-1 | 7.750e-1 |
| gsta4 | 47 | exemplar | 0.95 | 0.00 | 4.68 | 13.41 | 8.61 | 8.00 | 5.15 | Mm00494803_m1* | 1.200e-2 | -8.230e-1 | 7.780e-1 |
| irf4 | 49 | regulator | 0.80 | 6.50 | 3.54 | 6.28 | 4.32 | 4.09 | 0.40 | Mm00516431_m1* | 3.250e-2 | -7.490e-1 | 1.009e+0 |
| traf1 | 49 | exemplar | 0.93 | 0.00 | 3.39 | 9.38 | 4.96 | 4.61 | 2.12 | Mm00493827_m1* | 7.840e-3 | -8.480e-1 | 1.579e+0 |
| mxd1 | 49 | regulator | 0.90 | 7.19 | 5.64 | 9.08 | 6.73 | 6.60 | 0.60 | Mm00487504_m1* | 4.890e-3 | -8.710e-1 | 1.176e+0 |
| 6332401o19rik | 55 | exemplar | 0.97 | 0.00 | 2.97 | 7.16 | 3.53 | 3.39 | 0.49 | Mm00844775_s1* | 8.740e-1 | 6.700e-2 | 1.250e+0 |
| nr1h3 | 55 | regulator | 0.91 | 4.88 | 4.70 | 10.65 | 5.75 | 5.46 | 1.29 | Mm00443451_m1* | 7.870e-2 | -6.540e-1 | 8.020e-1 |
| ppp1r14c | 58 | exemplar | 0.94 | 0.00 | 2.90 | 7.30 | 4.35 | 3.86 | 1.13 | Mm00652462_m1 | 1.080e-1 | -6.110e-1 | 8.210e-1 |
| fosb | 59 | regulator | 0.88 | 8.28 | 2.69 | 10.63 | 5.59 | 3.80 | 8.33 | Mm00500401_m1* | 2.150e-3 | -9.030e-1 | 1.195e+0 |
| egr3 | 59 | regulator | 0.92 | 8.68 | 4.06 | 8.27 | 5.65 | 4.92 | 1.80 | Mm00516979_m1* | 2.170e-3 | -9.020e-1 | 8.220e-1 |
| hspa1b | 59 | exemplar | 0.95 | 0.00 | 3.01 | 11.40 | 6.83 | 5.57 | 7.46 | Mm03038954_s1* | 2.310e-1 | -4.780e-1 | 8.180e-1 |
| klf6 | 59 | regulator | 0.93 | 8.67 | 5.91 | 10.90 | 8.12 | 7.50 | 2.32 | Mm00516184_m1* | 1.170e-3 | -9.210e-1 | 1.235e+0 |
| fmnl2 | 62 | regulator | 0.73 | 5.04 | 3.80 | 9.72 | 8.02 | 8.27 | 1.24 | Mm00549621_m1 | 6.200e-2 | -6.830e-1 | 9.860e-1 |
| ampd3 | 62 | exemplar | 0.85 | 0.00 | 2.61 | 8.11 | 6.59 | 6.81 | 1.16 | Mm00477495_m1* | 1.150e-3 | -9.210e-1 | 1.156e+0 |
- Type indicates if the candidate is an exemplar (most higly correlated with module profile), or one of the top three predicted regulators, or a control gene.
- Correlation is the Pearson correlation of the candidate gene expression with the module profile in the microarray data.
- Z.score: the CLR-calculated Z-score representing the significance of the association between the candidate regulator and module.
- Minimum, Maximum, Mean, Median, and Variance: Range, center and variance estimates for the gene expression from the microarray data.
- TaqMan.ID: Assay identifier used in the Fluidigm experiments.
- MA/Flu Correlation: Pearson correlation between the biological-grouped averaged microarray expression values and Fluidigm deltaCts.
- Efficiency: TaqMan efficiency estimate as determined by estimating the slope of a standard curve with six ten fold dilutions of a pooled sample and the formula: effeciency = 10^(-1/slope)-1.
